成人免费网站在线 I 亚洲经典视频在线观看 I 亚洲91精品 I 人人91 I 亚洲黄色短视频 I 国产成人无码av I 女性高潮视频 I 色女综合 I 性欧美69 I 欧美国产日韩在线观看成人 I 亚洲精品第五页 I 欧美另类videosbestsex日本 I 色综合激情网 I 冲田杏梨av I 男女操网站 I 日韩久久一区二区三区 I 91在线视频精品 I 北条麻妃在线一区 I 成色视频 I 亚洲熟女乱综合一区二区 I 欧亚一区二区三区 I 青青在线视频一区二区三区 I 精品国产一区二区亚洲人成毛片 I 成人久久18免费网站漫画 I 国产69精品久久久久777 I 亚洲色大网站www永久网站 I 国产欧美一区二区三区不卡高清 I 国产影片中文字幕 I 性感美女福利视频网站 I 精品久久久久久久久妇女 I 久久成人网站亚洲综合 I 天天操天天天 I 亚洲高清最新av网站 I 奇米影视亚洲狠狠色 I 丝袜美腿美女一区二区 I 欧美日韩一区二区三区在线观看免费 I 日本高清色www在线安全 I 久久人人做人人妻人人玩精品hd I 午夜少妇在线观看视频

sigma現(xiàn)貨代理Dispase II D-4693

Description

Analysis Note

The enzyme can be initially dissolved in 50 mM Hepes/KOH pH 7.4, 150 mM NaCl at 10 mg/ml. Further dilutions should be made in the cell culture media most appropriate for the cells being dissociated. Dispase is a metalloproteinase and is activated by divalent cations including Ca2⁺ , Mg2⁺, Mn2⁺ and Fe2⁺.

Preparation Note

A 50 U/mL stock solution may be prepared by dissolving the powder in a buffer containing 10 mM NaAc (pH 7.5) and 5 mM CaAc. It should be stored at 4 °C.

Unit Definition

One unit will hydrolyze casein to produce color equivalent to 1.0 μmole (181 μg) of tyrosine per min at pH 7.5 at 37 °C (color by Folin-Ciocalteu reagent), unless otherwise indicated.

Physical form

lyophilized powder containing calcium acetate and milk sugar

Application

The enzyme from Sigma has been used in developing a protocol for ex vivoculture of mouse embryonic mammary buds.^[5] It has been used in the treatment of rat heart pieces during the isolation of mitochondria from rat heart.^[6] It has also been used for the isolation of dental pulp stem cells (DPSCs) by enzymatic hydrolysis. These cells have been compared with DPSCs isolated by explant method to analyse their stem cell and differentiation properties.^[1]
Dispase II has been used for Fluorescence-Activated Cell Sorting (FACS).^[3] It has also been used for separating visceral yolk sac layers.^[2]

Biochem/physiol Actions

Dispase II is a neutral protease that hydrolyzes the N-terminal peptide bonds of non-polar amino acid residues. It may be used for separating many tissues and cells grown in vitro. The enzyme is very gentle and does not damage cell membranes. It can also be used to prevent clumping in suspension cultures. This protease cleaves fibronectin and type IV collagen, but not laminin, type V collagen, serum albumin, or transferrin.^[4] Dispase II is specific for the cleavage of Leucine-Phenylalanine bonds. Ca²⁺, Mg²⁺, Mn²⁺, Fe²⁺, Fe³⁺ and Al³⁺activate the enzyme. EDTA, EGTA, Hg²⁺ and other heavy metals inhibit the enzyme activity.^[7] The enzyme contains 1g-atom of zinc per g-mol of purified enzyme. If this zinc component is removed by chelating agents such as EDTA or EGTA, an inactive apoenzyme is obtained. The enzyme is not inhibited by serum.

sigma現(xiàn)貨代理Dispase II D-4693

我公司代理sigma公司試劑，大量現(xiàn)貨，和選購